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New Presentation: Next generation risk assessment for consumer safety of cosmetics: A case study approach

New Presentation: Next generation risk assessment for consumer safety of cosmetics: A case study approach
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The principles for the use of non-animal approaches in consumer safety risk assessments were recently outlined by the International Cooperation on Cosmetics Regulation (ICCR). A key principle is that modern risk assessments should be exposure led. This means that novel methods that are used to make decisions […]

New Presentation: Mitochondrial toxicity and oxidative stress: Defining the tipping point between adaptive and adverse effects for consumer safety risk assessment

New Presentation: Mitochondrial toxicity and oxidative stress: Defining the tipping point between adaptive and adverse effects for consumer safety risk assessment
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Within emerging Next Generation Risk Assessment (NGRA) frameworks, oxidative stress and mitochondrial toxicity are included as early key events in several Adverse Outcome Pathways (AOPs). A key challenge is to understand how dose-response information from non-animal approaches to studying cell stress/mitochondrial toxicity can be integrated with consumer […]

New Presentation: High-throughput and non-depletive quantification in 3D liver microtissue in-vitro assay

New Presentation: High-throughput and non-depletive quantification in 3D liver microtissue in-vitro assay
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Non-animal approaches for assessing toxicological and environmental risk of ingredients require more advanced and realistic in-vitro models as well as a better understanding of a chemicals distribution and fate in-vitro and in-vivo. Here we applied a novel ultra-thin SPME fiber sampling method to analyse the fate of […]

New Publication: Flutamide-induced hepatic cell death and mitochondrial dysfunction involves inhibition of Nrf-2 mediated heme oxygenase 1 induction

New Publication: Flutamide-induced hepatic cell death and mitochondrial dysfunction involves inhibition of Nrf-2 mediated heme oxygenase 1 induction
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Flutamide is a widely used nonsteroidal anti-androgen for prostate cancer therapy, but its clinical application is restricted by the concurrent liver injury. Increasing evidence suggests that flutamide-induced liver injury is associated with oxidative stress, though the precise mechanism is poorly understood. Nuclear factor erythroid 2-related factor 2 […]

New Presentation: Understanding biotransformation of slowly cleared chemicals in in-vitro hepatic models

New Presentation: Understanding biotransformation of slowly cleared chemicals in in-vitro hepatic models
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Early prediction of clearance in human is often challenging, in particular for the growing number of low-clearance compounds. Widely used microsomes or hepatocytes suspensions only allow short term metabolism studies due to drastically declined the activity of drug metabolizing enzyme (i.e. in hepatocyte assay the activity declines […]

New Presentation: Retrofitting an Estrogen Receptor Transactivation Assay with Metabolic Competence Using Alginate Immobilization of Metabolic Enzymes (AIME)

New Presentation: Retrofitting an Estrogen Receptor Transactivation Assay with Metabolic Competence Using Alginate Immobilization of Metabolic Enzymes (AIME)
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The VM7Luc4E2 estrogen receptor (ER) transactivation assay is an OECD approved method (TG 457) for the detection of ER agonists and antagonists, and is also part of the Tox21 high-throughput screening (HTS) portfolio. Despite international acceptance as a screening assay, immortalized cell lines such as VM7Luc4E2 which […]

New Presentation: Challenges in Developing systems models for safety decision making

New Presentation: Challenges in Developing systems models for safety decision making
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The development of NGRA approaches will require a combination of both in vitro experimental work and in silico modelling. The talk looks at a few of these areas where the challenges facing safety decision making can be supported by modelling and informatics approaches with examples of previous […]

New Publication: High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes

New Publication: High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes
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In vitro high-throughput non-depletive and non destructive determination of concentrations of chemicals in biological matrices is of growing interest in numerous fields of clinical and bioanalytical studies. Some of the primary challenges facing analysts and researchers include the limited available volume of many biofluid samples, the requirement […]

New Publication: Using Transition State Modelling to Predict Mutagenicity for Michael Acceptors

New Publication: Using Transition State Modelling to Predict Mutagenicity for Michael Acceptors
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This paper describes of the use of Computational Chemistry methods to help in the prediction of toxicity endpoints by modelling the molecular initiating event. Read the publication here.

New Poster: Computational approaches for predicting Molecular Initiating Events

New Poster: Computational approaches for predicting Molecular Initiating Events
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Through our collaboration with University of Cambridge, we have used several in silico methodologies to investigate MIEs using chemical information. Structural features, reactivity patterns and artificial intelligence can be used to make predictions and gain greater understanding of why chemicals lead to toxicological effects. Quantum mechanical calculations […]